Modes of Growth in Mammalian Cells

Clonal Growth of Mammalian Cells in Vitro

Two methods for simple and rapid plating of single HeLa cells, human, carcinomatous cells, are described. These result in growth and formation of colonies from each single cell. One of these procedures uses irradiated, non-multiplying "feeder" cells to condition the medium. The second requires more gentle handling of the cells, but otherwise is virtually the same as that used in plating bacteri...

Growth factors for mammalian cells in culture.

Essentially all cultured animal cells require for growth1 animal serum or some other biological preparation. An understanding of the nature of the contributions made by these complex mixtures is of great interest for many reasons. Included are the desirability of better defined media for biochemical and other studies, the elucidation of growth requirements not detectable in experiments with who...

Molecular Growth Requirements of Single Mammalian Cells

The molecular nutritional requirements exhibited by mammalian cells can vary with the following conditions: whether the cells are plated singly or in massive inoculum; whether a clonal strain or a mixture of different genotypes is employed; whether the macromolecular fraction of serum used as a supplement was previously exposed to prolonged dialysis or other procedures which may release bound m...

Expression of Biologically Active Recombinant B-Domain-Deleted Human Factor VIII in Mammalian Cells

Growth modes of quasicrystals.

The growth of quasicrystals, i.e., aperiodic structures with long-range order, seeded from the melt is investigated using a dynamical phase field crystal model. Depending on the thermodynamic conditions, two different growth modes are detected, namely defect-free growth of the stable quasicrystal and a mode dominated by phasonic flips which are incorporated as local defects into the grown struc...